Lake Superior State University
Lake Superior State University
 
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Alum Success

Leslie Machado

Leslie Machado

LSSU Chemistry Major

PhD in chemistry at Johns Hopkins University

College of Natural and Mathematical Sciences

School of Physical Sciences

Environmental Analysis Laboratory

EAL Brochure (pdf)

EAL 2014 Price List (pdf)

Note: Discounts apply when more than 50 samples are submitted for each method. Additional discounts available for client prepared samples. Sample containers provided at no additional cost.

 

Inorganics

 

ICP-MS

Instrumentation includes:

  • Agilent 7500a ICP-MS housed in a class 100 clean room

  • Perkin-Elmer Analyst 300 Atomic Absorption Spectrometer

  • CEM Mars microwave digestor

  • Benchtop HEPA hood systems

 

Analysis

Method

Sample/Size

Metals by AAS SM 3111 Water / 0.1 L
Metals by ICP/MS EPA 6020 Solids / l.0 g
Sample Digestion Varies by Matrix Solids / 1.0 g (dry)
Total Mercury  EPA 1631 E Water / Solid

 

 

 

Microscopy

 

SEM

 

Instrumentation includes:

  • JEOL JSM 6100 Scanning Electron Microscope

 

Analysis

Sample/Size

Imaging Call for Quote
Qualitative EDS Elemental Composition Call for Quote

 

 

Wet Chemistry

 

AQ 1

Instrumentation includes:

  • Dionex DX500 Ion Chromatograph

  • Perkin Elmer Lamba 35 Double Beam UV/Vis Spectrophotometer

  • Perkin-Elmer Analyst 300 AA with Flame and Graphite Furnace

  • Seal Analytical AQ1 - Automated Discrete Analyzer

 

Analysis
Method
Sample/Size
Alkalinity SM 2320 B Water/ 0.1 L
Ammonia EPA 350.1 Water/ 0.1 L
Anions (F, Cl, Br, NO2, NO3, o-PO4, and SO4) EPA 300 Water/ 0.1 L
Biological Oxygen Demand (BOD) SM 5210 B Water/ 0.1 L
Chlorophyll (a,b, & c) SM 10200 H Water/ l.0 L
Conductivity SM 2510 B Water/ 0.1 L
Enterococci (MPN) SM 9230 D Water/ 0.1 L
Hardness SM 2340 B Water/ 0.2 L
Nitrate SM 4500 Water/ 0.1 L
Nitrate + Nitrite SM 4500 Water/ 0.1 L
ortho-Phosphate SM 4500P F Water/ 0.2 L
pH SM 4500H+ Water/ 0.1 L
Silica SM 4500-SiO2 D Water/ 0.1 L
Total coliforrn/E coli (MPN) SM 9223 Water/ 0.l L
Total Dissolved Solids SM 2540 C Water/ 0.2 L
Total Kjeldahl Nitrogen EPA 351.2 Water/ 0.l L
Total Phosphorus SM 4500P Water/ 0.2 L
Total Suspended Solids SM 2540 D Water/ 0.2 L
Turbidity EPA 180.1 Water/ 0.2 L

 

 

  
Organics

Environmental Analysis

Instrumentation includes:

  • Agilent 6890N Series/5973 Series GC/MS

  • Hewlett-Packard 5890 Series II/5971 Series GC/MS

  • Agilent 6890 Series GC/FID

  • Archon Purge and Trap & Teledyne Tekmar Velocity XPT Purge

Gas Chromatography

 

Analysis
Method
Sample/Size
Organics by GC/FID EPA 8015 C Water / l.0 L
Organics by GC/MS EPA 8270 D Water / l.0 L
Organics by Purge and Trap GC/MS EPA 8260 C Water / l.0 L
Sample Extraction Varies by Matrix Water/Solids
Note: For availble compounds please call.

 

 

Cyanotoxin

 

Instrumentation includes:

  • Waters 2695 HPLC PDA/Fluorescence

  • Molecular Devices SpertraMax M2e Mircoplate Reader

  • Bio-Tek Instruments FLx 800 Fluorescence Microplate Reader

  • VirTis Benchtop K Lyopholizer

 

High Performance Liquid Chromatography/Photo Diode
Array (HPLC/PDA)

 

Analysis
Sample/Size
Extra-cellular priority cyanotoxins Water / 0.5 L
Total priority cyanotoxins Water / 0.5 L
Note: Priority toxins include Anatoxin-a, Cylindrospermopsin, and Microcystin RR, LR, LA, YR, and LF.

 

 

Enzyme Linked Immunosorbant Assay (ELISA)

 

Analysis
Sample/Size
Microcystin Water / 0.0l L
Cylindrospermopsin Water / 0.0l L
Saxatoxin Water / 0.0l L

 

 

Phytoplankton

 

cyanobacteria

Cyanobacteria Identification/Enumeration

 

Analysis
Method
Sample/Size
genus level WHO
Water / 0.l L

 

 

PCR

 

qPCR

 

Instrumentation includes:

  • Applied Biosystems StepOne Plus qPCR

  • Applied Biosystems Thermocycler

qPCR

 

Analysis
Method
Sample/Size
Enterococcus
EPA 1611
Water / 0.5 L
Human Bacteroides
DRAFT EPA Method C
Water / 0.5 L

Impact in the real world...

Rachel Claucherty-Arnold

Rachel Claucherty-Arnold
Environmental Health

What made Rachel's Lake Superior State experience so unique was the practical research she did with top-notch faculty.

"I really enjoyed working with environmental chemistry professor Judy Westrick and biologist Deb Stai," Rachel says. "For my senior project, we evaluated a lab method for cultivating a fungus that causes infections in humans."

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