Lake Superior State University
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Alum Success

"I chose to transfer to Lake Superior State University because of the personal attention I would receive. I did not want to attend a large university where I would be a number lost in the masses. The well-rounded curriculum allowed me to obtain my position at John Deere. Having a good balance of theory and hands-on knowledge is critical for my position and Lake State gave me a good foundation in these skills."

Maria DiValentin, Mechanical Engineering 2001
Field Service Representative,
John Deere Power Systems
Waterloo, IA

School of Engineering & Technology

Senior Projects 2009-2010

Meet this year's project teams:

Team AIR logo
Team AIR

Automation Innovation Renovation
Electronic and computer engineering aspects of new robotics line.

2009-10 Team DRIVE
Team DRIVE

Dynamic Response Integration for Vehicle Engineering
Test stand for American Axle & Manufacturing

2009-10 Team FIRE logo
Team FIRE

Future Innovators of Railroad Engineering
Scale prototype coupler for "O" and "HO" scale model railroads (second phase) .

2009-10 Team I2 logo
Team I2

Industrial Innovations
Automated work cell for loading and unloading laser etchers

2009-10 Team MTD logo
Team MTD

Modern Technology Development
Work with the Prototype Development Center in areas of design and building prototype systems and/or products.

2009-10 Team PAS logo
Team PAS

Precision Automation Solutions
Mechanical aspects of the new robotics line.

Character- ization of Yellow Pigments in Freshwater Flavo- bacteria

Ashley Ryckman

The yellow color of Flavobacteria is due to the presence of carotenoids and flexirubin-type pigments. These complex chemical structures have shown to produce antioxidant properties, as well as antibacterial activity. The KOH test and reverse-phase high performance liquid chromatography (HPLC) was used to characterize carotenoid and flexirubin pigment production in three strains of Flavorbacteria: FR 87, FR Y, and FR 93. Optimization of pigment and separation was performed by quant-prep HPLC. Separation of four pigment fractions from each strain was attained using semi-prep HPLC. Antibacterial activity of the pigment fractions was tested using a MTT Assay. Fexirubin biosynthesis genes, darA and darB, of related pigment producing Flavobacteria were used to compare flexirubin pigments in FR 87. The three strains were determined to produce the carotenoid, Zeaxanthin. FR 87 produced 13 flexirubin pigments, FR Y produced 14 flexirubin pigments and FR 93 produced 20 flexirubin-type pigments. It was determined that compounds in all three strains demonstrated antibacterial activity. The flexirubin biosynthesis gene, darA, is 89.9% similar to Flavobacterium johnsoniae, and 73.2% similar to Flavobacterium psychrophilum.

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