Lake Superior State University
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Alum Success

"It seems like the more people I talk to, the more I realize just how good of an engineering program LSSU has. I appreciate your classes, your ability to make learning an enjoyable experience, and the hands-on attitude of LSSU as a whole. I am very glad I chose LSSU."

Jake Weinmann Controls Engineer, ADD Software/Fanuc Robotics

Computer Engineering

Computer engineering combines
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Computer engineer Manar Wadi poses with the controller she adapted to run a plasma cutter in one of Lake Superior State University's manufacturing technology labs. The senior project capped four years of hard work at Lake Superior State for Wadi, who works for PATEL, a telecommunications company, in her home town of East Jerusalem.

"I really wanted to see if I could successfully bridge two cultures while keeping my own solid identity," says Manar. "I wanted to help myself, as well as others around me, overcome barriers of culture and prejudgment that separate us. This was one major goal of my college experience, and LSSU offered a perfect environment to do this.

Being a woman engineer doesn't create any obstacles or awkwardness whatsoever to me pursuing a career in Palestine or any of the Arab countries in the Middle East," she says. "In fact, I just received a job offer by Etisalat in Dubai. This shows that women professionals do have job opportunities in the Middle East."

  
  • Applied Manufacturing Technologies
  • Ab Inito
  • Bauer Controls
  • Boeing
  • Continental Automotive, Inc.
  • Crockett Technologies
  • Delaney Software Services
  • Edgewood Chemical Biological Center
  • Electrical & Computer Engineering / Purdue
  • JR Automation
  • PALTEL (Palestine Telecom)
  • and many more!
Many Options

Graduates may choose from engineering positions ranging from computer systems design, software or hardware development to research, robotics and applications.

Optim- ization of Salmon DNA as an Internal Standard for qPCR

Elaina Murray

The Escherichia coli species is a human fecal contamination indicator and as such is used in beach monitoring efforts. Quantifying E. Coli presence in local beach waters helps the health department determine if a beach should be closed. The current method of determination, Colilert, takes 18 hours to produce data. Quantitative Polymerase Chain Reaction (qPCR), which measure genetic DNA, is also method used to quantify the number of E. Coli, but it can be done much faster than Colilert. In order to standardize the qPCR results, an internal standard is included which is salmon DNA. This project goes through the process of optimizing the salmon standard curve. Each of the components was modified and the resulting standard curve was analyzed for improvements; the primers and probe were purchased new and the concentrations were varied, the DNA was purchased new and the standard curve concentrations and dilution methods were varied, the DNA was cleaned with a Qiagen kit, and new master mix and bovine serum albumin were purchased and prepared. We found that changes to the concentrations of primers and probe and cleaning the DNA showed an increase of optimization, and that changing the dilution methods had no effect of optimization. A combination of the above modifications may be able to produce an optimized salmon DNA standard curve.

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