Lake Superior State University
Lake Superior State University
 
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Alum Success

“I chose LSSU expecting a very good engineering education. What I didn’t expect was faculty with real-world engineering experience and abilities, labs with real-world equipment, projects with real-world outcomes, and an entire campus staff with real interest in my success, as a student and yet today. My LSSU engineering education has created or supported every desired career opportunity. LSSU was absolutely the right place for me.”

Dan Goodrich,
Mechanical Engineering 1999,
Vehicle Test & Development,
Electronic Brake Systems Group

Mechanical Engineering

LSSU is 1 of only 3 universities in the U.S. with a robotics specialization at the undergraduate level
Faculty and Staff
Jon Coullard


Jon Coullard

Laboratory Engineer
BS 1990, Lake Superior State University

 

Jim Devaprasad


Jim Devaprasad

Professor, Manufacturing Engineering Technology
Program Chair, Manufacturing Engineering Technology
Director, Robotics Laboratory
Coordinator, Senior Projects
BS 1983, University of Madras, India
MS 1986, University of New Mexico

 

Paul Duesing

Paul Duesing

Associate Professor
Mechanical Engineering
Coordinator, Cooperative Education
BSME 1971, MSME 1973, University of Michigan
Licensed professional engineer for Michigan and Ohio

 

Robert Hildebrand


Robert Hildebrand

Assistant Professor
Mechanical Engineering
B.S.E. (ME) 1990, University of Michigan
M.S. (EM) 1994, University of Nebraska
Ph.D. 2001, Royal Institute of Technology (KTH) Sweden

Muhammad Janjua


Muhammad Janjua

Assistant Professor
Mechanical Engineering
B.S. 2000, Ghulam Khan Institute of Engineering and Technology
M.S. 2003, City College of New York
Ph.D. 2009, New Jersey Institute of Technology

 

David Leach


David Leach

Engineering Projects Manager

 

Sai Nudurupati


Sai Nudurupati

Assistant Professor
BS 2003, Chaitanya Bhasathi Institute Of Technology; PhD 2009, New Jersey Institute of Technology

 


  

"I just landed my dream job!  I'm moving to Connecticut where I will be designing high-performance racing mountain bike suspensions.  My interest in biking is the reason studied mechanical engineering in the first place and I just wanted to let Professor Duesing know that his ability to teach the fundamentals made it possible for me to reach my goal."

--Jesse Jakomait '03
Cannondale Bicycle Corp.

 

"I am thankful for the project management skills I learned from Professor McDonald and the dedication to detail and quality that Professor Duesing required from us. I would not be where I am now if it was not for their valuable influence."

--Brian Carnecki
Stryker,
Inventory Control Supervisor

Optim- ization of Salmon DNA as an Internal Standard for qPCR

Elaina Murray

The Escherichia coli species is a human fecal contamination indicator and as such is used in beach monitoring efforts. Quantifying E. Coli presence in local beach waters helps the health department determine if a beach should be closed. The current method of determination, Colilert, takes 18 hours to produce data. Quantitative Polymerase Chain Reaction (qPCR), which measure genetic DNA, is also method used to quantify the number of E. Coli, but it can be done much faster than Colilert. In order to standardize the qPCR results, an internal standard is included which is salmon DNA. This project goes through the process of optimizing the salmon standard curve. Each of the components was modified and the resulting standard curve was analyzed for improvements; the primers and probe were purchased new and the concentrations were varied, the DNA was purchased new and the standard curve concentrations and dilution methods were varied, the DNA was cleaned with a Qiagen kit, and new master mix and bovine serum albumin were purchased and prepared. We found that changes to the concentrations of primers and probe and cleaning the DNA showed an increase of optimization, and that changing the dilution methods had no effect of optimization. A combination of the above modifications may be able to produce an optimized salmon DNA standard curve.

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