Lake Superior State University
Lake Superior State University
 
Related Sites

 

Alum Success

Video at ABC.com

Ariel Kelly, a Park and Recreation graduate who is a park ranger at Yosemite National Park, was interviewed as part of a program feature that highlights beautiful places in America.

Parks & Recreation

Parks & Recreation Management challenges the mind and body
<
Alumni
 
Recent Graduates

Our graduates succeed!

"The education I received at LSSU has provided me with a solid foundation on which to start building my current educational goals.  I am looking forward to being able to combine my skills as a recreational therapist and as a teacher in working with special education students.  Thank-you with providing me with such a good educational experience that has well prepared me for graduate school." 

--Kate Marie Gerlach-Brooks

 

 


  
  • Brandon Fast has accepted a position with Benzie County, MI.
  • Denise Neahusan, has been hired as a parks worker for Aurora, CO.
  • Deborah Stille is employed as a Park Ranger at Michigan's Sliver Lake State Park .

 

   
Recent Graduates in the Spring 2014 Newsletter
   
School of Recreation Studies and Exercise Science School of Recreation Studies and Exercise Science Spring 2014 Newsletter (pdf)

Optim- ization of Salmon DNA as an Internal Standard for qPCR

Elaina Murray

The Escherichia coli species is a human fecal contamination indicator and as such is used in beach monitoring efforts. Quantifying E. Coli presence in local beach waters helps the health department determine if a beach should be closed. The current method of determination, Colilert, takes 18 hours to produce data. Quantitative Polymerase Chain Reaction (qPCR), which measure genetic DNA, is also method used to quantify the number of E. Coli, but it can be done much faster than Colilert. In order to standardize the qPCR results, an internal standard is included which is salmon DNA. This project goes through the process of optimizing the salmon standard curve. Each of the components was modified and the resulting standard curve was analyzed for improvements; the primers and probe were purchased new and the concentrations were varied, the DNA was purchased new and the standard curve concentrations and dilution methods were varied, the DNA was cleaned with a Qiagen kit, and new master mix and bovine serum albumin were purchased and prepared. We found that changes to the concentrations of primers and probe and cleaning the DNA showed an increase of optimization, and that changing the dilution methods had no effect of optimization. A combination of the above modifications may be able to produce an optimized salmon DNA standard curve.

Apply Today!

Home » Parks & Recreation > Alumni
Share this page with your friends: